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AUPR The Matter Laboratory Cancer Cells & Proteolytic Enzymes Discussion

AUPR The Matter Laboratory Cancer Cells & Proteolytic Enzymes Discussion

AUPR The Matter Laboratory Cancer Cells & Proteolytic Enzymes Discussion

For your final assignment of Online Part 3, you will design your own hypothetical experiment and write a short prompt to describe how you would go about conducting such an experiment from start to finish, including how you would grow cells, how to prepare them for RNA-seq, and what you would do after you have obtained raw gene counts. Get creative with this assignment – its your chance to think like a scientist in a real-world scenario.

If you need a general idea of how a prompt should look, I have attached a short sample prompt:

i need 2 different answers for the same question

. attached the sample to look at

Please submit a minimum 300 word PDF or Word document depicting your experiment by Sunday 5/2 at 11:59 PST.

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Online Part III Assignment 3 Sample Response I work in a microbial bioengineering lab which focuses on production of metabolites with high-added value. Our lab currently has established media on which our microbe grows; however, we have 5 experimental growth substrates which we predict can give us better yields, in comparison to our standard media. The way I would approach this scenario is growing our bacterial strain, in triplicate, on our standard media and all 5 experimental growth substrates. I would ensure all other factors remain static (growth time, growth temperature, amount of biomass used to inoculate) and harvest cells at the end of their incubation. After harvesting cells, I would extract mRNA using an established kit and prepare samples for cDNA library construction/RNA sequencing. After I receive my raw FASTQ reads,